![]() To begin, weigh about five grams of lyophilized Spirulina platensis cells or other cyanobacterium cells containing only chlorophyll a or chlorophyll-a in thylakoid membranes and use a mortar and pestle to crush it. While preparing the following stock solutions perform all chlorophyll preparation steps under a chemical hood under green light or in the dark to minimize photo damage.Īlways add nitrogen or argon before freezing the pigments for storage and ensure that all solvents are analytical grade. Demonstrating the procedure will be Dominica Bednarczyk, a post-doc in my research group. Thus, it is well-suited for screening assays. The main advantage of this technique is that it does not require tagging or immobilizing the proteins. This method enables assembly of chlorophylls with recombinant proteins, which allows rigorous studies of chlorophyll-protein interactions and opens up new possibilities for constructing novel chlorophyll-protein complexes.
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